Rat HSPA5/GRP78/BiP ELISA Kit

Assay Genie offers a Sandwich ELISA kit for the quantitative measurement of HSPA5 (also known as GRP78/BiP) in rat-derived samples. HSPA5 is a molecular chaperone involved in protein folding and the cellular stress response, and its expression is reported to be upregulated in conditions including cancer, diabetes, and neurodegenerative diseases, supporting its use as a biomarker in related research.

The kit is configured as a double-antibody Sandwich ELISA with colorimetric TMB detection. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The manufacturer reports a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Unopened kits are stored at 2-8°C for 12 months.

Published synonyms for the target include Hspa5, GRP-78, Grp78, Steroidogenesis-activator polypeptide, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, and BiP, with Uniprot identifier P06761. Recovery values reported by the manufacturer average 95% for serum, 97% for EDTA plasma, and 90% for heparin plasma.