Rat HSP-90 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative detection of Heat Shock Protein 90 (HSP-90) in rat samples. The double-antibody sandwich assay uses a colorimetric readout and is reactive with rat serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. The manufacturer describes HSP-90 as a key protein in cellular stress response and protein folding, with dysregulation implicated in cancer, neurodegenerative disorders, and cardiovascular diseases, making it a biomarker of interest for studies into disease pathogenesis and potential therapeutic targeting.

Stated performance figures include a sensitivity of 75 pg/mL and an assay range of 125-8000 pg/mL. Reported linearity across 1:2, 1:4 and 1:8 dilutions (n=5) is 85-101%/86-99%/88-99% for serum, 90-100%/88-97%/81-100% for EDTA plasma, and 85-102%/88-99%/82-93% for heparin plasma. Spike-and-recovery averages are 93% for serum (range 86-104%), 96% for EDTA plasma (range 88-103%), and 94% for heparin plasma (range 86-101%). The associated Uniprot entry is P82995. Listed synonyms include HSP90AA1, Hsp90, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2, FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1 alpha, and heat shock 90kD protein 1 alpha-like 4.

The protocol covers reagent and TMB equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding at 37°C for 60 minutes, HRP-streptavidin (SABC) binding at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance reading at 450 nm immediately after stop solution addition. Recommended storage is 2-8°C for 12 months, with a third-party listing also citing +4°C conditions for storage and shipping.