Rat HSD11B1 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA, double antibody kit is designed for the quantitative measurement of 11-beta-hydroxysteroid dehydrogenase type 1 (HSD11B1) in rat samples. The kit supports research into corticosteroid metabolism, glucocorticoid regulation, and conditions linked to HSD11B1 dysregulation, including metabolic disorders, inflammation, and stress-related disease. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance characteristics include a sensitivity of 0.938 ng/mL and an assay range of 1.563-100 ng/mL. Linearity has been evaluated in serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, ranging from 82-105% across all conditions. Recovery averages 96% for serum, 95% for EDTA plasma, and 98% for heparin plasma. Detection is colorimetric, employing a biotin-labeled detection antibody, HRP-streptavidin conjugate, and TMB substrate, with absorbance read at 450 nm.

The kit is stored at 2-8°C for 12 months. The associated UniProt entry is P16232, and the target is also known by synonyms including 11-DH, 11-beta-HSD1, HSD11, HDL, HSD11B, and HSD11L. The research area is metabolism.