Rat Hormone-sensitive lipase/LIPE ELISA Kit

From Assay Genie, this rat-reactive sandwich ELISA employs a double antibody format with colorimetric detection to quantitatively measure Hormone-Sensitive Lipase (LIPE). The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is reported to have a sensitivity of 18.75 pg/mL with a detection range of 31.25-2000 pg/mL. Reagents are stored at 2-8°C for up to 12 months.

LIPE is a critical enzyme in lipid metabolism that plays a key role in the breakdown of stored fat for energy production, with dysregulation linked to metabolic disorders such as obesity and diabetes, making it a relevant target for metabolism research. The kit is intended to support studies of LIPE in metabolic pathways, identification of related biomarkers, and exploration of novel treatment strategies.

The assay procedure begins with equilibration of reagents and TMB substrate to room temperature and setup of standard, test, and zero-control wells on the pre-coated plate. This is followed by primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, and HRP-Streptavidin (SABC) binding at 37°C for 30 minutes. TMB substrate is then added and incubated in the dark for 10-20 minutes, the reaction is stopped, and absorbance is read at 450 nm. Sample preparation includes allowing blood to clot and centrifuging serum at 1000 × g for 20 minutes, collecting plasma with anticoagulant tubes and centrifuging at 1000 × g for 15 minutes at 2-8°C, homogenising tissue in PBS with protease inhibitors, centrifuging cell culture supernatant at 2500 rpm for 5 minutes, and lysing cells with lysis buffer containing protease inhibitors.