Rat Homovanillic acid (HVA) ELISA Kit

A competitive ELISA kit from Assay Genie intended for the quantitative determination of homovanillic acid (HVA), a metabolite of dopamine that plays a role in neurotransmission within the central nervous system. The manufacturer states that HVA levels have been linked to neurological disorders including Parkinson's disease, schizophrenia, and attention deficit hyperactivity disorder (ADHD), supporting its use in research into neurotransmitter metabolism and the pathophysiology of these conditions.

The assay uses a competitive ELISA format with a stated measurement range of 0.78-50 ng/mL and is supplied for research use only. Documented sample preparation protocols cover serum, plasma (collected with EDTA or heparin as anticoagulant), urine, cerebrospinal fluid, cell culture supernatant, cell lysates, tissue homogenates, tissue lysates, and breast milk. The colour reaction is terminated with stop solution and the optical density of each well is measured on a micro-plate reader set to 450 nm.

Kit reagents comprise a dismantlable ELISA microplate (8×12 strips), a lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate solution, stop solution, and plate sealers. Storage temperatures are itemised at -20°C for the plate, standard, diluents and detection reagents, and 4°C for the wash buffer, substrate, stop solution and plate sealers.