Rat HMG-CoA Reductase/HMGCR ELISA Kit

Designed by Assay Genie for the quantitative measurement of HMG-CoA Reductase (HMGCR) in rat samples, this Sandwich ELISA kit employs a double-antibody method with colorimetric detection. The kit is intended for research applications relating to cholesterol biosynthesis, where HMGCR serves as a key regulatory enzyme. Dysregulation of HMGCR activity has been linked to cardiovascular disorders and metabolic syndromes, making accurate quantification relevant to studies of cholesterol regulation and potential therapeutic interventions.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay demonstrates a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Linearity is supported across dilution factors of 1:2, 1:4, and 1:8 for serum (92-105%, 88-99%, 80-100%), EDTA plasma (86-105%, 82-96%, 84-96%) and heparin plasma (85-105%, 91-101%, 82-99%). Recovery averages 95% in serum (n=5), 94% in EDTA plasma, and 96% in heparin plasma. Reagents should be stored at 2-8°C for up to 12 months. The procedure involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance measurement at 450 nm following stop solution addition.