Rat Histone H3 (Hist2h3c2) ELISA Kit (RTFI01475)

Quantitative measurement of the Hist2h3c2-encoded histone variant in rat specimens is supported by this double-antibody immunoassay from Assay Genie. Researchers investigating chromatin remodeling, nucleosome turnover, and epigenetic regulation in rat models can use the kit to track H3 abundance across treatment cohorts, including experiments probing replication stress and DNA damage response. A 50 uL sample volume is used for serum-type or plasma-type specimens, while cell culture supernatant or tissue lysate requires 100 uL per well. Specificity testing shows the antibody pair selectively recognizes Histone H3 with no obvious cross reaction with other analogues. Each kit ships with a pre-coated microplate, calibrated recombinant standards, detection antibody, chromogenic substrate, and a complete protocol sufficient for 96 determinations per plate. Reagents should be stored refrigerated between runs, protected from light, and aliquoted to limit freeze-thaw cycles.