Rat High Affinity Choline Transporter (ChT) ELISA Kit

The Rat High Affinity Choline Transporter (ChT) ELISA Kit from Assay Genie enables quantitative measurement of ChT protein in rat samples, supporting neuroscience research focused on cholinergic signaling. ChT, encoded by the SLC5A7 gene, mediates sodium-dependent choline uptake at presynaptic terminals and acts as a rate-limiting step in acetylcholine biosynthesis relevant to studies of memory, cognition, and neurodegenerative disease models.

The plate-based immunoassay uses a capture antibody pre-coated on a microplate and a conjugated detection reagent to generate a measurable signal proportional to ChT concentration. Researchers typically prepare brain tissue homogenates from regions such as hippocampus, cortex, or striatum, then load 100 µL of diluted sample per well. The kit includes standards, detection reagents, wash buffer, and substrate sufficient for a full plate run.

Store unopened components refrigerated; reconstituted standards should be aliquoted and kept frozen at -20°C for extended shelf life. Allow reagents to equilibrate to room temperature before use, and protect chromogenic substrate from light. Intended for research use only in vitro applications such as quantifying transporter expression following pharmacological treatment, gene knockdown, or in rat models of aging and Alzheimer's-type pathology.