Rat Heparanase (HPA) ELISA Kit

This kit from Assay Genie enables quantitative measurement of heparanase (HPA) levels in laboratory rat specimens, supporting research in extracellular matrix biology, angiogenesis, and tumor microenvironment investigation. The HPA target protein plays a well-characterized role in heparan sulfate proteoglycan cleavage, making the assay relevant to studies of cell invasion, inflammatory cascades, and metastatic progression in rodent models.

Each package supplies reagents for 96 individual determinations on a pre-coated microplate, with an absorbance-based read-out. The assay has been validated for use with rat-derived matrices including serum-type specimens, plasma-derived samples, and tissue homogenate supernatants from organs such as kidney, liver, and spleen. Researchers studying diabetic nephropathy, fibrotic tissue remodeling, or cancer cell dissemination in rats can use this product as a biomarker quantification tool in vitro.

Components include the capture-antibody-coated plate, standard calibrator prepared from recombinant rat heparanase, detection antibody working solution, substrate, wash buffer, and stop reagent. Reagents should be stored refrigerated between uses, and the standard should be aliquoted to limit freeze-thaw cycles. The workflow follows standard ELISA steps: incubate, wash, develop, stop, and read absorbance. Results are interpolated from a standard curve generated in each run. Intended for research use only.