Rat Hedgehog Homolog, Sonic (SHH) ELISA Kit

Sonic Hedgehog (SHH) is a secreted morphogen that patterns the neural tube, limb buds, and other embryonic structures during rat embryogenesis, and continued hedgehog pathway activity influences adult cell fate decisions and tissue homeostasis. Researchers working in developmental biology, neuroscience, and oncology rely on quantitative SHH measurement to characterize signaling in disease models, neural progenitor differentiation, and regenerative response following injury. This ELISA kit from Assay Genie supports those investigations by providing a microplate-based colorimetric assay validated for rat specimens.

The reagents accommodate measurement in tissue homogenates and cell lysates prepared from dissected rat tissues or cultured cells. Samples are typically clarified by centrifugation or filtration before being applied to the plate for binding and detection, with absorbance read on a standard plate reader. Each kit ships as a complete set including the microplate, capture antibody, detection antibody, recombinant SHH standard, and buffer reagents sufficient for one full run.

Reaction steps are performed at room temperature during the assay workflow. Reconstituted standards and antibody working solutions should be kept refrigerated to preserve activity until use. Intended for laboratory research use only.