Rat Heat Shock Protein Beta 6 (HSPb6) ELISA Kit

Beyond the listed specifications, a few biological and technical details improve experimental planning with this Assay Genie kit. HSPB6 (Hsp20) runs at approximately 17 kDa as a monomer and assembles into dynamic oligomers that redistribute during cellular stress; it is phosphorylated at Ser16 by PKA and PKG, and the phosphorylated form docks with 14-3-3 proteins to drive smooth muscle relaxation and cardiomyocyte survival signaling.

• Rat-specific capture antibody with no significant cross-reactivity with HSPB1 (Hsp27) or HSPB5 (alphaB-crystallin)
• Validated for cardiac ischemia-reperfusion and ischemic preconditioning studies in rat left ventricular and aortic tissue
• Homogenize in buffer with protease and phosphatase inhibitor cocktails, aliquot clarified lysates, and store at -80 degrees C prior to the assay to preserve phosphorylation-dependent epitopes
• Use the 10 to 0.156 ng/mL standard series to interpolate unknowns within the linear portion of the curve