Rat HCY (Homocysteine) ELISA Kit (RTFI01470)

Homocysteine (HCY) is a sulfur-containing amino acid derivative whose elevated serum levels serve as a well-established biomarker in cardiovascular disease research, renal dysfunction studies, and investigations of B-vitamin metabolism. This competitive ELISA from Assay Genie enables quantitative determination of HCY in rat biological matrices.

Each kit is supplied with a precoated strip plate, lyophilized standards, detection antibody, substrate solution, and wash buffer sufficient for 50 μL sample volumes run in duplicate.

Typical protocols reach completion in approximately 2.5 hours at room temperature, with absorbance read on a standard microplate reader. Reagent components should be stored refrigerated at 4°C and protected from light to preserve enzymatic activity of the conjugate. Researchers should aliquot working solutions to limit repeated freeze-thaw cycles of the antibody reagents.

Widely applied in studies of hyperhomocysteinemia, atherosclerosis modeling, diabetic nephropathy, and neurodegenerative disease research, the kit supports translational investigations into methyl group donor metabolism and one-carbon cycle biochemistry. For research use only; not validated for diagnostic procedures.