Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit (RTES01107)

A colorimetric sandwich ELISA from Assay Genie for rat Glutathione Peroxidase 1 (GPX1), with an assay time of 3 h 30 min. The kit reports a sensitivity of 18.75 pg/mL and a detection range of 31.25-2000 pg/mL, and is designed for measuring GPX1 levels in rat serum, plasma, lysate samples and cell culture supernatants.

Samples supported include serum, plasma and other biological fluids, with a sample volume of 100 μL. Reported recovery is 80%-120%. The manufacturer states that the kit recognizes rat GPX1 and that no significant cross-reactivity or interference between rat GPX1 and analogues was observed. Listed research areas are cancer, cell biology, metabolism and signal transduction.

Supplied components include a dismountable micro ELISA plate, reference standard, sample diluent, biotinylated detection antibody, HRP conjugate, assay diluents, wash buffer, substrate reagent, stop solution, plate sealer and a technical manual; storage is at 2-8°C. GPX1 is described as an important antioxidant enzyme that protects cells from oxidative damage by reducing hydrogen peroxide and lipid peroxides, and its dysregulation has been implicated in cancer, inflammatory conditions and neurodegenerative disorders.