Rat Glypican 3 (GPC3) ELISA Kit

Glypican-3 (GPC3) is a glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycan that regulates Wnt, Hedgehog, and FGF signaling pathways during embryogenesis and tissue repair. Scientists working on hepatocellular carcinoma, hepatic regeneration, and germ cell tumor biology use this Sandwich ELISA to quantify circulating GPC3 concentrations in serum, plasma, and tissue homogenates from rat preclinical models. The kit from Assay Genie uses a rat-specific pair of capture and detection antibodies with no significant cross-reactivity with rat Glypican-1 or Glypican-2, supporting selective measurement in lipid raft and extracellular matrix studies. Recommended sample volume is 50 uL per well, with all reagents supplied in a complete kit for in vitro research use only. Store components refrigerated, and avoid repeated freeze-thaw cycles of reconstituted standards. The protocol is widely cited in hepatic disease research, biomarker discovery, and developmental biology across academic and pharmaceutical laboratories.