Rat Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) ELISA Kit

Glycerolipid and triglyceride biosynthesis begins at the outer mitochondrial membrane, where glycerol-3-phosphate acyltransferase (GPAM) catalyses the first committed acylation step, converting glycerol-3-phosphate to lysophosphatidic acid. This rat-specific ELISA Kit from Assay Genie quantifies the enzyme in tissue homogenates through an absorbance-based microplate workflow, supplied in a 96-assay format with pre-coated wells, calibrated standards, biotinylated detection antibody, streptavidin-HRP conjugate, and TMB substrate.

Because GPAM sits upstream of phospholipid and triglyceride synthesis, this ELISA is commonly deployed in hepatic steatosis models, insulin-resistance studies, and dietary or pharmacological interventions that shift hepatic lipid partitioning. The validated sample matrix is tissue homogenates, typically prepared from liver, epididymal adipose, or quadriceps skeletal muscle, where GPAM expression is most often interrogated in metabolic research. Measuring enzyme abundance directly in tissue rather than serum or plasma lets investigators link GPAM levels to organ-level metabolic state, and the kit is configured for in vitro research use only.