Rat Glutathione S Transferase Theta 2 (GSTt2) ELISA Kit

Quantitative measurement of rat glutathione S-transferase theta 2 (GSTT2) protein levels across blood-derived fractions, tissue homogenates, cell lysates, and cell culture supernatants supports toxicology and xenobiotic-metabolism research. The kit from Assay Genie employs a matched antibody pair immobilized on a microplate, providing absorbance-based readout on a standard plate reader.

GSTT2 catalyzes conjugation of reduced glutathione to electrophilic substrates, contributing to phase II detoxification of drugs, environmental chemicals, and endogenous compounds. Investigators studying hepatic biotransformation, oxidative-stress responses, chemoprotective pathways, and species-specific susceptibility to toxicants apply this kit to evaluate enzyme expression in preclinical rat models.

Reagents are provided in vials and include a pre-coated microplate, lyophilized recombinant rat GSTT2 standards, biotinylated detection reagent, streptavidin-HRP conjugate, assay diluent, wash buffer, chromogenic substrate, and stop solution, supplied for research use only. The workflow is carried out at room temperature with refrigerated storage recommended for kit components and prepared reagents between runs to preserve activity.