Rat Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit

This double-antibody ELISA from Assay Genie enables researchers to measure Glutathione S-Transferase Alpha 3 (GSTa3) levels in rat-derived specimens, supporting toxicology, hepatic function, and xenobiotic metabolism studies. The kit uses an enzyme-mediated absorbance readout on a standard microplate, accommodating up to ninety-six determinations per run.

Rat GSTa3 belongs to the alpha class of cytosolic glutathione S-transferases, enzymes that conjugate reduced glutathione to electrophilic substrates for phase II detoxification. Investigators studying chemical-induced liver injury, drug metabolism, or oxidative stress pathways rely on quantitative GST isoform profiling to characterize tissue-specific responses in rodent models.

Compatible specimen types include blood-derived fractions, tissue homogenates, cell lysates, and spent culture media, providing flexibility across in vivo and in vitro experimental designs. Reconstituted reagents should be stored refrigerated, and unused strips returned to a sealed pouch with desiccant to preserve antibody activity across the workflow.

Each Assay Genie kit ships with a pre-coated microplate, lyophilized standards, detection antibody, assay diluent, wash concentrate, substrate solution, and stop reagent. Standard curves are generated from serial dilutions of the supplied calibrator, with absorbance read at the wavelength appropriate for the substrate provided.