Rat Glutamine synthetase (GS) ELISA Kit

Glutamine synthetase (GS) is the ATP-dependent ligase that converts glutamate and ammonia into glutamine, anchoring nitrogen homeostasis, hepatic ammonia clearance, and neurotransmitter recycling across mammalian tissues. The Rat Glutamine synthetase (GS) ELISA Kit from Assay Genie provides researchers with an absorbance-based quantitative readout of GS abundance in rat samples, supporting studies in liver metabolism, astrocyte and Müller-cell biology, retinal function, and skeletal-muscle physiology where glutamine flux is a central metabolic indicator.

The kit ships as a complete microplate workflow containing pre-coated wells, calibrated recombinant standards, a biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and stop reagent. The package supports 96 individual determinations, allowing standard curves and large experimental cohorts to be processed in parallel. Validated sample matrices include serum samples, plasma samples, freshly prepared tissue homogenates, and clarified cell lysates from rat donors.

Standard operating procedure: dilute standards and unknown samples per the enclosed protocol, dispense 100 µL per well onto the pre-coated microplate, incubate, wash, develop with the chromogenic substrate, and record absorbance on a standard plate reader. Refrigerate all reagents on arrival, allow the plate and buffers to reach room temperature before use, shield the substrate from light, and minimize repeated freeze-thaw cycles of reconstituted standards.