Rat Glutaminase (GLS) ELISA Kit

The glutaminase enzyme, encoded by the GLS gene, catalyzes the hydrolytic deamidation of glutamine to glutamate and ammonia, a reaction that gates glutamine entry into the tricarboxylic acid (TCA) cycle and supports de novo glutamate biosynthesis. Because dysregulated glutamine catabolism is a hallmark of proliferating cells and has been linked to tumor growth, neurotoxicity, and renal ammoniagenesis, quantifying GLS protein abundance in rodent tissues and fluids is a recurring need in metabolic, oncologic, and neuroscience laboratories. The Rat Glutaminase (GLS) ELISA Kit from Assay Genie is configured as a quantitative immunoassay intended to measure GLS levels in rat-derived samples, supporting studies of hepatic glutamine handling, brain glutamine-glutamate cycling, and tumor xenograft or syngeneic rodent models of glutaminolysis. The kit ships with the reagents required to run a standard quantitative protein-detection workflow and is intended for research use only in laboratory settings. Reagents should be kept refrigerated upon arrival, with standards and conjugates handled to minimize freeze-thaw cycles and protect antibody activity. This product serves investigations of glutamine metabolism across rat tissue homogenates and other relevant biological matrices, including work that compares GLS expression between control and experimental animal cohorts.