Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

The 73 kDa catalytic subunit of glutamate-cysteine ligase, the rate-limiting enzyme in glutathione biosynthesis, is a key biomarker for research into cellular redox status, oxidative stress responses, and xenobiotic detoxification. This colorimetric immunoassay from Assay Genie is configured for quantitative measurement of the catalytic subunit in rat-derived specimens, supporting studies of hepatic, renal, pulmonary, and neurological disease models in which altered glutathione metabolism has been implicated.

The kit is supplied in a 96-assay microplate format and accepts liquid samples drawn from blood (serum or plasma), prepared tissue homogenates, and cell lysates, accommodating both in vivo and in vitro experimental designs. Investigators focused on Nrf2-mediated antioxidant gene regulation, chemically induced glutathione depletion, or recovery from oxidative insult can use the assay to monitor catalytic-subunit expression alongside downstream glutathione pools.

Assay Genie supplies all reagents needed for the colorimetric readout on a standard plate reader. Components should be refrigerated and handled according to the included protocol to preserve reagent activity. The kit is intended for research use only and is not validated for diagnostic procedures.