Rat GLUT4/Glucose Transporter 4 ELISA Kit

Designed for the quantitative measurement of Glucose Transporter 4 (GLUT4) in rat samples, this Sandwich ELISA from Assay Genie uses a double-antibody format with colorimetric detection. The kit is intended for research applications exploring glucose metabolism, insulin resistance, diabetes, and related metabolic disorders, where GLUT4 acts as a key regulator of glucose uptake in muscle and adipose tissues.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Manufacturer-quoted performance characteristics include a sensitivity of 0.375 ng/mL and an assay range of 0.625–40 ng/mL. Reported linearities for serum (n=5) are 89–98% at 1:2, 85–104% at 1:4, and 90–101% at 1:8; for EDTA plasma (n=5), 82–94%, 82–101%, and 83–97%; for heparin plasma (n=5), 80–94%, 81–96%, and 95–99%. Spike-and-recovery averages are 94% (serum), 100% (EDTA plasma), and 92% (heparin plasma).

The protocol follows a standard sandwich workflow: primary incubation at 37°C, biotinylated detection antibody binding, HRP-streptavidin (SABC) conjugation, TMB substrate development, and absorbance measurement at 450 nm. Reagents are stored at 2–8°C for 12 months. The associated UniProt entry is P19357, and the kit is categorised within the Signal Transduction research area.