Rat Glucose Transporter 4 (GLUT4) ELISA Kit

This Assay Genie kit follows a multi-step protocol in which samples and recombinant rat GLUT4 standards are pipetted into antibody-coated wells, incubated with a biotinylated detection reagent, and developed with streptavidin-HRP and TMB substrate. The full workflow generally requires 3 to 4 hours of bench time, with several wash and incubation stages optimized for low background. The kit is commonly applied to monitor insulin-stimulated glucose uptake in L6 myotubes, 3T3-L1 adipocytes, and isolated rat soleus or EDL muscle preparations, and to evaluate pharmacological responses to AMPK activators, thiazolidinediones, and exercise-mimetic compounds in rodent models of type 2 diabetes and obesity. Reproducibility is supported by intra-assay precision reported as %CV below 8% and inter-assay values below 10%. Reconstituted standards should be aliquoted into single-use vials to limit freeze-thaw exposure, while detection reagents are diluted on the day of the run and protected from light. The plate layout includes dedicated wells for a seven-point standard curve plus blanks, allowing quantitative interpolation of unknown samples against a freshly prepared dilution series.