Rat Glucose Transporter 3/GLUT3 ELISA Kit

This high-quality Sandwich ELISA Kit from Assay Genie provides colorimetric detection of Glucose Transporter 3 (GLUT3) levels in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format, offers a sensitivity of 0.188 ng/mL, and operates over a range of 0.313-20 ng/mL. Components are stored at 2-8°C for 12 months.

Validated linearity in serum ranges from 88-105% (1:2), 84-99% (1:4) and 80-100% (1:8); in EDTA plasma from 91-97%, 88-100% and 87-100%; and in heparin plasma from 88-105%, 82-100% and 80-98%. Recovery averages 93% for serum, 91% for EDTA plasma, and 95% for heparin plasma. The protocol involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding, HRP-streptavidin (SABC) binding, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm.

GLUT3 is a key protein that facilitates glucose transport across cell membranes, essential for energy metabolism and cellular function. Abnormalities in its expression have been linked to metabolic disorders including diabetes and obesity, making it a valuable biomarker for studying these conditions and potential therapeutic interventions in rat model systems.