Rat Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit

Quantify Glucose-6-Phosphatase, Catalytic subunit (G6PC) levels in rat-derived specimens with this colorimetric ELISA kit from Assay Genie. G6PC catalyzes the terminal step of gluconeogenesis and glycogenolysis, making the assay a relevant tool for studies of hepatic glucose output, insulin resistance, and metabolic disease models in rodents.

Each kit is configured in a 96-assay microplate format and uses a colorimetric readout, allowing a full plate of standards and unknowns to be processed in a single run. The assay is validated for serum-based samples, plasma preparations, and tissue homogenates, giving researchers the flexibility to pair blood-based and tissue-based endpoints within the same study.

Reagents are intended for research use only and should be kept refrigerated between uses to preserve antibody activity. The workflow follows a standard quantitative immunoassay format: pre-coated capture plate, sequential addition of standards or samples, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate development, stop solution, and absorbance reading.

Common applications include endocrinology research, hepatic physiology, type 2 diabetes models, and metabolic phenotyping of rat cohorts where G6PC expression serves as a biomarker of hepatic glucose production.