Rat Glucagon PharmaGenie ELISA Kit (SBRS0135)

Endocrine and metabolic researchers working with rat models can use the Assay Genie Rat Glucagon PharmaGenie ELISA Kit to quantify glucagon levels in cell culture supernatants and serum samples. Built around a competitive immunoassay principle, the kit is configured for 96 individual determinations and produces a colorimetric readout on a standard microplate reader, removing the need for radioactive or fluorescent reagents.

It supports both in vivo and in vitro research workflows investigating pancreatic islet biology, glucose homeostasis, metabolic disease, diabetes, and counter-regulatory hormone signaling, enabling the measurement of glucagon secretion from alpha cells under varying physiological conditions. Each kit includes a pre-coated plate, lyophilized standards, assay buffer, detection antibody, conjugate, substrate, stop solution, and a detailed protocol manual.

The competitive format is particularly effective for capturing small peptide hormones like glucagon, which can be difficult to detect using two-antibody methods due to the hormone's compact molecular size. All reagents are intended for research use only and should be handled according to the included protocol. The kit ships with enough material to run the full plate in duplicate, supporting the generation of a standard curve and multiple sample replicates for reliable quantitation.