Rat GHRL (Ghrelin) ELISA Kit (RTES00707)

Assay Genie supplies this competitive, colorimetric ELISA for measuring GHRL (ghrelin) in rat serum, plasma and other biological fluids, using a stated sample volume of 50 μL. Reactivity is rat, and the manufacturer reports that the kit recognizes rat GHRL with no significant cross-reactivity or interference between rat GHRL and analogues.

Specifications listed by the manufacturer are a sensitivity of 0.1 ng/mL, a detection range of 0.16-10 ng/mL, an assay time of 2 h 30 min and recovery of 80%-120%. Detailed recovery results are 85-101% with an average recovery of 92% in EDTA plasma and 93-106% with an average recovery of 98% in cell culture media. Precision results give intra-assay CV values of 6.1%, 4.63% and 6.85% and inter-assay CV values of 6.16%, 8.07% and 6.93%.

The kit comprises a dismountable micro ELISA plate, reference standard and sample diluent, concentrated biotinylated detection antibody, concentrated HRP conjugate, detection antibody and HRP conjugate diluents, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Storage is 2-8°C. Listed research areas are cancer, cardiovascular, metabolism, neuroscience and signal transduction.