Rat Ghrelin (GHRL) ELISA Kit

Ghrelin (GHRL) is a 28-amino acid acylated peptide hormone predominantly secreted by X/A-like (P/D1) cells of the gastric fundus. The octanoylation of serine-3 by ghrelin-O-acyltransferase is essential for biological activity at the growth hormone secretagogue receptor (GHSR1a), positioning ghrelin as a central regulator of appetite, energy balance, and growth hormone release. This Assay Genie rat-specific kit applies a competitive immunoassay approach suited to short peptide analytes, with chromogenic signal generation appropriate for in vitro research workflows.

The kit supports quantification of endogenous ghrelin across diverse sample matrices, providing a tool for research into metabolic disorders, obesity, cachexia, gastric bypass physiology, and neuroendocrine regulation. Each kit ships as a complete research-use-only package containing pre-coated microplate wells, lyophilized standards, detection reagents, wash buffer, substrate solution, and stop reagent in individually sealed vials.

Ghrelin immunoassay workflows benefit from rapid sample handling to preserve acylation status; plasma should be processed with protease inhibitors and acidification where acyl-ghrelin preservation is required. The competitive ELISA format distinguishes this kit from sandwich-format assays and is the preferred method for short peptide hormones with limited epitope space. Reagents should be stored refrigerated between uses, and frozen aliquots used to limit freeze-thaw cycles of standards and samples.