Rat GDF6 ELISA Kit

For the precise measurement of growth differentiation factor 6 (GDF6) in rat samples, this Sandwich ELISA kit from Assay Genie offers a double antibody sandwich format with colorimetric detection. GDF6, also referred to as BMP-13, is a member of the bone morphogenetic protein (BMP) family and plays a role in skeletal development and joint formation. The kit is suitable for serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples.

The kit delivers a sensitivity of 0.938 ng/mL and a quantitative range of 1.563-100 ng/mL. Linearity is 86-105% for serum, 83-96% for EDTA plasma, and 82-95% for heparin plasma at a 1:2 dilution, with recovery ranging from 88-104% for serum, 88-99% for EDTA plasma, and 89-101% for heparin plasma. Reagents are stored at 2-8°C for 12 months. The procedure includes a 90-minute primary incubation at 37°C, followed by detection antibody binding, HRP-streptavidin binding, and TMB substrate development, with absorbance read at 450 nm.