Rat GDF15 (Growth Differentiation Factor 15) CLIA Kit (RTES00269)

The Rat GDF15 CLIA Kit from Assay Genie is a chemiluminescent sandwich immunoassay built on a 96-well microplate format, enabling quantitative measurement of Growth Differentiation Factor 15 in serum, plasma, and other biological fluids with greater sensitivity and a broader dynamic range than conventional colorimetric ELISA.

GDF15 (also called MIC-1, NAG-1, or PTGFB) is a divergent member of the TGF-β superfamily and a recognized biomarker of mitochondrial stress, inflammation, and metabolic dysfunction. Investigators studying obesity, cachexia, cardiovascular injury, renal disease, and oncology use this kit to track circulating GDF15 in rat preclinical models.

Key specifications:

• Detection method: chemiluminescence
• Sample volume: 50–100 µL per well
• Assay runtime: approximately 3–4 hours at room temperature
• Standard curve covering low pg/mL to high ng/mL concentrations
• Storage: 2–8 °C; protect from light; avoid repeated freeze-thaw cycles
• For in vitro research use only

Each kit includes a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, assay buffers, wash concentrate, and chemiluminescent substrate. Assay Genie supplies a detailed protocol and full technical support to ensure reproducible intra-assay and inter-assay CV performance across runs.