Rat GDF11 ELISA Kit

From Assay Genie, this Sandwich ELISA, Double Antibody kit enables quantitative measurement of Growth Differentiation Factor 11 (GDF11) in rat samples. The assay is reactive with rat, with a stated sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit is stored at 2-8°C for 12 months.

GDF11 is described as a key regulator of cell growth, differentiation, and development, with roles in tissue regeneration, muscle maintenance, and aging, making it a significant biomarker for physiological and pathological research. The target corresponds to Uniprot entry Q9Z217 and is also known by the synonyms BMP-11, Bone morphogenetic protein 11, and GDF-11.

Reported linearity (n=5) at 1:2, 1:4, and 1:8 dilutions: serum 90-97%, 84-95%, 81-92%; EDTA plasma 85-105%, 84-92%, 88-98%; heparin plasma 88-101%, 89-98%, 84-100%. Reported recovery ranges and averages (n=5): serum 87-100% (94%), EDTA plasma 91-99% (94%), heparin plasma 86-97% (90%). The procedure uses a TMB substrate with absorbance measured at 450 nm.