Rat GCK/ Glucokinase ELISA Kit

Assay Genie offers a Sandwich ELISA kit built on a double-antibody format for the quantitative measurement of Glucokinase (GCK) in rat samples. The colorimetric assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting metabolism-focused research into glucose regulation and conditions such as diabetes.

The kit reports a sensitivity of 0.375 ng/mL across a working range of 0.625–40 ng/mL, with reagents stored at 2–8°C for 12 months. The workflow uses a biotin-labelled detection antibody with HRP-Streptavidin (SABC) and a TMB substrate, with absorbance read at 450 nm. Reported recovery averages 95% for serum, 96% for EDTA plasma and 92% for heparin plasma, while dilution linearity across 1:2, 1:4 and 1:8 falls between 80% and 104% for the same matrices.