Rat Gastrin PharmaGenie ELISA Kit (SBRS0131)

The Assay Genie competitive immunoassay provides quantitative measurement of gastrin in rat-derived samples using a pre-coated microplate. Each kit includes a gastrin standard curve, antibody-coated wells, detection reagents, and substrate solution for color development. The assay relies on competitive binding, where endogenous peptide hormone in the sample competes with coated antigen for limited antibody sites, enabling precise quantification of circulating gastrin concentrations. Researchers apply this kit to gastrointestinal and metabolic studies examining gastric acid secretion regulation, G-cell activity in the antral mucosa, and hormonal responses to dietary or pharmacological challenges in rodent models. The protocol uses 50 uL of sample per well, with intra-assay precision below 10%. Reconstituted standards should be handled cold and the microplate equilibrated to room temperature before initiating the run, while unused strips are returned to refrigerated storage in the foil pouch with desiccant.