Rat Gastrin (GT) ELISA Kit

Each Assay Genie Rat Gastrin (GT) ELISA Kit is supplied with a pre-coated microplate and carries a documented 12-month shelf life when stored refrigerated. The gastrin target corresponds to UniProt entry P04563 and is recognized under synonyms including GAST, GAS, big gastrin, and gastrin components I, II, and III. Matrix recovery has been validated for serum (80-102%, average 91%), EDTA plasma (81-99%, average 90%), and heparin plasma (80-89%, average 84%), with serial dilution linearity ranging from 79 to 104%. Assay precision is documented at intra-assay CV <10% and inter-assay CV <12%, and kit activity loss remains below 5% through the expiration date under appropriate storage. The protocol uses sequential 1 hour incubations at 37°C with detection reagent A and detection reagent B, separated by wash steps, followed by a 15-25 minute TMB substrate incubation at 37°C and a 50 µL stop solution before absorbance measurement. Loss rate testing confirms stable performance when unopened vials are kept refrigerated, supporting consistent batch-to-batch results across the kit’s working life.