Rat Gas 1 PharmaGenie ELISA Kit (SBRS1639)

GAS1 (Growth Arrest-Specific 1) is a GPI-anchored cell-surface glycoprotein that halts proliferation in G0/G1 and acts as a competitive antagonist of Sonic Hedgehog. The Assay Genie Rat GAS1 PharmaGenie ELISA Kit delivers a quantitative colorimetric readout for this target in rat-derived samples, read on a standard microplate reader, and is positioned for research into liver regeneration, neural tube closure, retinal development, hedgehog-driven tumorigenesis, and apoptosis in rat model systems.

Supplied as a complete kit with a pre-coated microplate, the assay accommodates 96 individual determinations and is validated for rat cell culture supernatants, plasma samples, and serum samples. Reconstituted reagents, working standards, and prepared samples should be handled according to the enclosed protocol insert, with standard ELISA wash and incubation steps applied.

Routine applications include profiling GAS1 expression in regenerating rat liver tissue following partial hepatectomy, characterizing cell-cycle arrest in primary rat hepatocyte cultures, and quantifying circulating GAS1 in rodent disease models. Investigators studying hedgehog pathway modulation, glial cell biology, and cancer cell line behavior in rat systems will find the format suitable for longitudinal sampling from individual animals, and the assay is intended for research use only.