Rat GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit (RTFI01451)

Researchers studying rat models can measure Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels quantitatively with this ELISA Kit from Assay Genie, with reactivity limited to rat specimens. The constitutively expressed housekeeping enzyme serves as a widely used loading control in Western blot normalization and gene expression studies, making quantitative measurement valuable for confirming equal protein loading across experimental lanes. This research-use-only immunoassay is formatted as a 96 Assays pack and includes the reagents required to construct a standard curve and to evaluate experimental samples in parallel. The enclosed protocol recommends running all determinations in duplicate to improve analytical precision. Practical applications extend to validation of sample integrity prior to downstream proteomic workflows, comparative assessment of housekeeping gene expression under varied physiological or pharmacological conditions, and quality control of lysate preparations derived from rat tissues. The kit pairs capture and detection antibodies against the target to produce a measurable signal proportional to GAPDH concentration in each well.