Rat GADD153/DDIT3 ELISA Kit

Assay Genie's Rat GADD153/DDIT3 ELISA Kit is a Sandwich ELISA, Double Antibody assay designed for quantitative measurement of GADD153 (also known as DDIT3) in rat samples. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting research into cellular stress responses relevant to cancer, diabetes, and neurodegenerative disorders within the Cell Death research area.

The assay has a sensitivity of 0.375 ng/mL and a working range of 0.625-40 ng/mL. Spiked recovery in serum (n=5) ranges from 86-100% with an average of 96%, EDTA plasma (n=5) from 92-100% with an average of 100%, and heparin plasma (n=5) from 90-105% with an average of 98%. Linearity across 1:2, 1:4, and 1:8 dilutions falls between 82-102% for serum, EDTA plasma, and heparin plasma. Unopened kits should be stored at 2-8°C for 12 months.