Rat FSTL1 (Follistatin Like Protein 1) CLIA Kit (RTES00467)

Assay Genie's chemiluminescent immunoassay for rat FSTL1 leverages an acridinium ester or luminol-based signal pathway to quantify this Follistatin-like protein 1 biomarker with sensitivity that surpasses standard colorimetric ELISA. FSTL1, also known as TSC-36 or FRP, is a secreted extracellular glycoprotein implicated in fibroblast activation, immune cell signaling, angiogenesis, and tissue remodeling cascades. In rat disease models, circulating FSTL1 concentrations are routinely monitored in studies of myocardial infarction, pressure-overload heart failure, bleomycin-induced pulmonary fibrosis, collagen-induced arthritis, sepsis, and tumor cachexia. The 96-well microplate is pre-coated with a monoclonal capture antibody specific to rat FSTL1, and the standard curve typically spans a dynamic range of approximately 7.8-500 pg/mL, enabling detection of low-abundance analyte in small-volume serum or plasma aliquots (often 50-100 µL per well). For best results, equilibrate all reagents and plates to room temperature before pipetting, perform thorough washes between incubation steps, and protect luminescent substrates from light exposure to preserve signal output. Researchers investigating inflammatory cytokine networks, extracellular matrix turnover, or cardiovascular remodeling pathways will find this CLIA format particularly suited to applications where higher analytical sensitivity is needed than conventional sandwich ELISA can deliver, and the kit is intended for research use only.