Rat Forkhead Box Protein P3 (FOXP3) ELISA Kit

This quantitative immunoassay kit from Assay Genie measures Forkhead Box Protein P3 (FOXP3), the defining transcription factor of CD4+CD25+ regulatory T cells (Tregs), in rat-derived samples. FOXP3 drives Treg lineage commitment and immunosuppressive function, making its protein-level quantification a routine readout in immunology, autoimmunity, and transplant research. Loss-of-function mutations in FOXP3 are linked to the scurfy phenotype and to IPEX syndrome in humans, which underscores why accurate protein quantification matters when characterizing Treg fitness in animal models.

Compatible samples include rat tissue homogenates and cell lysates, covering spleen, thymus, lymph node, and peripheral blood mononuclear cell preparations commonly used to characterize Treg abundance. The assay runs on a microplate with a colorimetric read-out, with each kit supplying reagents for 96 individual determinations in a breakable well plate configuration that lets unused strips be reserved for later runs.

Researchers pair this protein readout with flow cytometry FOXP3 staining and FOXP3 mRNA qPCR to correlate surface marker expression, transcript levels, and total protein. Published applications include rat models of experimental autoimmune encephalomyelitis, collagen-induced arthritis, inflammatory bowel disease, and graft-versus-host disease, where shifting Treg numbers report on immune regulation status.

Reagents are supplied in a pack format intended for refrigerated storage between uses, and unopened components remain stable through the manufacturer-stated shelf life. Each lot is quality-controlled for rat-specific reactivity, and the kit is for research use only.