Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit

FOXO1 is a key transcriptional effector of the insulin/IGF-1 signalling pathway, regulating genes involved in hepatic gluconeogenesis, lipid metabolism, oxidative stress responses, and autophagy. Quantifying FOXO1 protein abundance in rat models is a routine step in metabolic disease research, including studies of type 2 diabetes, insulin resistance, hepatic steatosis, and ageing biology, where changes in total FOXO1 levels or nuclear localisation often reflect altered PI3K-Akt pathway activity.

This colorimetric immunoassay kit from Assay Genie is configured specifically for rat-derived samples. It is used with tissue homogenates prepared from rat liver, skeletal muscle, adipose depots, pancreatic islets, and brain regions, as well as with cell lysates from cultured rat cell lines and primary hepatocytes. The kit is supplied for research use only and is not intended for diagnostic applications or clinical decision-making.

The microplate-based format runs 96 determinations per plate, supporting standard curve-based quantification across multiple treatment groups, time points, and biological replicates on a single plate. Users should follow the supplied protocol for buffer preparation, recommended sample dilutions, and standard curve construction. Store the kit refrigerated and protect reagents from light to preserve activity through the stated shelf life.