Rat FNDC5/Fibronectin type III domain-containing 5 ELISA Kit

A high-quality Sandwich ELISA from Assay Genie, designed for the accurate measurement of Fibronectin type III domain-containing 5 (FNDC5) levels in rat samples. The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and employs a colorimetric double-antibody sandwich detection method.

With a sensitivity of 37.5 pg/mL and an assay range of 62.5-4000 pg/mL, the kit delivers reproducible quantitative data. Recovery performance across matrices shows an average of 95% for serum (range 88-104%), 93% for EDTA plasma (range 88-102%), and 91% for heparin plasma (range 85-98%). Linearity data for serial dilutions at 1:2, 1:4 and 1:8 are: serum 86-102%, 89-101%, 88-104%; EDTA plasma 83-99%, 84-101%, 82-100%; heparin plasma 80-99%, 82-100%, 80-98%, all tested with n=5.

FNDC5, also known as Irisin, FRCP2, and Fibronectin type III repeat-containing protein 2 (UniProt Q8K3V5), is a key regulator of metabolism and energy expenditure that has been implicated in metabolic disorders such as obesity and diabetes, making this kit a useful tool for research in these areas. Reagents should be stored at 2-8°C for up to 12 months.