Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit

This solid-phase immunoassay from Assay Genie is configured to quantify Fibronectin Type III Domain Containing Protein 5 (FNDC5) in rat research specimens. FNDC5 is a transmembrane precursor that, upon proteolytic cleavage, releases the myokine irisin into circulation, linking skeletal muscle activity with thermogenic regulation and energy expenditure in adipose tissue.

Each kit supplies reagents sufficient for 96 individual determinations read by an absorbance-based colorimetric readout suitable for laboratory research on energy balance, mitochondrial biogenesis, exercise physiology, and obesity-related pathways. The protocol accommodates a variety of sample matrices relevant to rodent studies, including tissue homogenates prepared from skeletal muscle and brown adipose depots, cell lysates from cultured myotubes, and conditioned media from differentiated adipocytes.

Standard laboratory ELISA workflows are supported, with appropriate incubation and washing steps performed on the included microplate. Researchers investigating UCP1 induction, glucose uptake modulation, or the muscle-fat endocrine axis can apply this kit to studies of irisin secretion dynamics in rat models of metabolic disease, exercise training, and nutritional intervention.

Storage recommendations and full protocol guidance are supplied with the kit, which is intended for research use only and is not approved for diagnostic or clinical procedures.