Rat Fibroblast Growth Factor 4 (FGF4) ELISA Kit

This quantitative immunoassay from Assay Genie is built around a microplate format and uses a paired-antibody configuration to measure endogenous Fibroblast Growth Factor 4 (FGF4) in rat-derived samples. FGF4 is a heparin-binding growth factor involved in embryonic patterning, limb bud development, tooth morphogenesis, and maintenance of pluripotent stem cells, so the kit finds its main applications in developmental biology, stem cell research, and regenerative medicine studies conducted on rodent models.

The assay accepts liquid samples and tissue homogenates prepared from rat sources, with researchers commonly using it for time-course studies of FGF4 expression during organogenesis, in tumor models where FGF4 acts as a mitogenic driver, and in stem cell differentiation protocols where FGF4 withdrawal is used to drive lineage commitment.

Reagents are supplied in individual vials within the kit, sufficient for one or more plate runs depending on the number of standards and replicates. Standard curves, wash buffer, detection antibody, and substrate components are organized to support simultaneous quantification of FGF4 across experimental and control wells, and the procedure can be performed on standard laboratory plate readers equipped for absorbance measurement at the wavelength specified in the manual.

Typical workflows run from sample incubation through detection and stop-step in roughly three to four hours at room temperature, and the kit is intended for research use only in laboratory settings such as academic, biotechnology, and pharmaceutical research environments.