Rat Fibroblast Growth Factor 3 (FGF3) ELISA Kit

FGF3 (Fibroblast Growth Factor 3), historically known as INT-2 in earlier literature, is a member of the fibroblast growth factor family that mediates epithelial-mesenchymal signaling during organ development. It has been investigated in studies of inner ear morphogenesis, branchial arch patterning, and tumor biology, where its chromosomal locus at 11q13 is amplified in certain carcinomas.

This quantitative immunoassay from Assay Genie enables measurement of rat FGF3 protein across biological sample matrices including blood-derived fluids and tissue homogenates. The assay uses paired antibodies and a colorimetric readout compatible with standard laboratory microplate readers, packaged as a complete 96 assays kit with all required reagents, recombinant standards, buffers, and pre-coated wells included for research workflows.

Each plate run supports construction of a standard curve alongside unknown samples, with duplicate wells recommended for both standards and unknowns to improve precision. Researchers commonly apply the kit to investigate FGF3 expression in rat tissues, conditioned cell culture media, and body fluids for studies of developmental biology, neural crest signaling, and oncogenic transformation pathways. Related applications include western blot validation, immunohistochemistry comparison studies, and targeted protein quantification in animal model systems.

For sample handling, aliquoting prior to storage and minimizing freeze-thaw cycles helps preserve the integrity of the target analyte. Reagent components are supplied in sealed vials and should be kept refrigerated upon arrival until first use, with remaining working solutions handled according to the supplied protocol insert. The kit is intended for in vitro research use only and is not validated for diagnostic procedures.