Rat Fibroblast Growth Factor 21 (FGF21) ELISA Kit

Fibroblast growth factor 21 acts as a metabolic hepatokine that regulates glucose uptake, fatty acid oxidation, and ketogenesis, and the Assay Genie Rat FGF21 ELISA Kit delivers quantitative colorimetric measurement of this hormone in rat-derived samples on a standard microplate format. The kit is configured for 96 assays and is intended for research use only, supporting studies of obesity, type 2 diabetes, NAFLD, and mitochondrial dysfunction where FGF21 serves as a circulating biomarker.

FGF21 is secreted primarily by hepatocytes in response to fasting, ketogenic diets, and cold exposure, where it acts on adipose tissue through FGFR1 in complex with the co-receptor β-Klotho to stimulate adiponectin release and thermogenic gene expression. Researchers studying the FGF21-adiponectin axis, hepatic endocrine signaling, or brown adipose activation routinely employ quantitative rat FGF21 measurements alongside metabolic phenotyping in rodent models.

The assay accepts a range of rat sample matrices including blood-derived fractions, tissue homogenates, cell lysates, and cell culture supernatants, and readouts are obtained on a standard microplate reader. Reagents are supplied in vials sufficient to complete the full plate; storage and handling instructions are detailed in the product insert accompanying the kit.