Rat Fibroblast growth factor 20 (Fgf20) ELISA Kit

This sandwich ELISA from Assay Genie quantitatively measures rat Fibroblast Growth Factor 20 (FGF20) in serum and plasma samples using a colorimetric microplate readout. The assay captures FGF20 between a pre-coated antibody and a biotinylated detection antibody, with signal development producing a measurable absorbance at 450 nm following TMB substrate addition and a stop solution.

FGF20 is a paracrine signaling protein of the FGF9 subfamily with documented roles in dopaminergic neuron survival, otic vesicle patterning, and renal branching morphogenesis, so quantitative detection supports neuroscience, developmental biology, and renal physiology studies in rat models. The 96-well microplate format supports up to 96 assays in duplicate when sample and standard curves are loaded, and a typical run is completed within a single working day.

Unopened kits should be stored at 2-8 °C; reconstituted standards and diluted detection antibody are typically frozen at -20 °C in single-use aliquots to limit repeated freeze-thaw cycles. Each lot is validated for sensitivity, intra-assay precision (typically CV below 10%), and minimal cross-reactivity with related FGF family members, supporting reproducible quantification across a working range commonly spanning low to mid pg/mL concentrations for serum and plasma matrices.