Rat Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

The Assay Genie Rat FGF2 ELISA Kit measures basic fibroblast growth factor (bFGF), a heparin-binding mitogen central to angiogenesis, wound repair, and stem-cell proliferation studies. The competitive format pairs a pre-coated microplate with biotinylated detection antibody and streptavidin-HRP to deliver an absorbance-based signal, with sensitivity reported at 46.875 pg/mL and a standard curve spanning 78.125 to 5000 pg/mL. Each kit ships with sufficient reagents for 50 uL sample volumes, including coated wells, lyophilized standards, detection antibody, HRP conjugate, wash concentrate, TMB substrate, and stop solution. Unopened components should be refrigerated; reconstituted standards must be frozen at -20°C in single-use aliquots to limit freeze-thaw degradation. Investigators in cardiac regeneration, neural plasticity, and tumor microenvironment research rely on this assay to quantify FGF2 release from cell culture supernatants and tissue homogenates under pharmacological stimulation. The full protocol completes in approximately 3 hours at room temperature, and validation studies show no significant cross-reactivity with rat FGF1, VEGF, or PDGF isoforms, supporting specific quantification of endogenous rat FGF2.