Rat FETUA (Fetuin A) ELISA Kit (RTES01076)

This Assay Genie sandwich ELISA measures FETUA in rat serum, plasma, and other biological fluids using a colorimetric detection method. Assay time is “4.5h,” and the required sample volume per well is “100µL.” Optical density is measured spectrophotometrically at “450 nm ± 2 nm,” and sample concentration is calculated by comparison with the standard curve.

Reported performance includes a detection range of “6.25-400 ng/mL” and sensitivity of “3.75 ng/mL.” The manufacturer reports that no significant cross-reactivity or interference between rat FETUA and analogues was observed. Listed validation data show average recovery of “98%” in serum, “99%” in EDTA plasma, and “100%” in cell culture media. Reported intra-assay CV values are “5.63%,” “5.47%,” and “3.72%,” while inter-assay CV values are “6.38%,” “5.07%,” and “5.24%.”