Rat Ferrochelatase (FECH) ELISA Kit

Ferrochelatase is the terminal enzyme of the heme biosynthesis pathway, catalysing the insertion of ferrous iron into protoporphyrin IX at the inner mitochondrial membrane. The Assay Genie Rat Ferrochelatase ELISA Kit quantitatively measures FECH protein in rat samples, validated for tissue homogenates and related preparations, through a colorimetric microplate immunoassay workflow. Each kit supplies reagents sufficient for 96 assays and is intended for research use only. The sealed kit should be kept refrigerated before first use to preserve shelf life, and reconstituted working solutions remain stable under cold storage. Researchers apply this assay to studies of porphyrin metabolism, erythropoietic protoporphyria, mitochondrial iron homeostasis, and hepatic heme synthesis disorders, as well as disease models linked to FECH deficiency in rodent systems.