Rat Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

The Assay Genie rat FABP3 ELISA kit quantifies Fatty Acid Binding Protein 3, also called H-FABP or heart-type fatty acid binding protein. This approximately 15 kDa cytosolic carrier protein is abundant in cardiac myocytes and skeletal muscle fibers, where it shuttles long-chain fatty acids toward mitochondrial beta-oxidation. In cardiovascular research, circulating H-FABP rises within hours after ischemic myocardial injury, making this assay valuable for time-course studies of cardiac damage, drug-induced cardiotoxicity, and heart failure in rat models. The colorimetric immunoassay accommodates diverse sample matrices including tissue homogenates, cell lysates, cell culture supernatants, and serum or plasma specimens from rat donors, supporting both in vivo and in vitro experimental designs. Each kit contains a pre-coated microplate, lyophilized reference standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, stop reagent, and assay diluent, providing reagents for 96 individual determinations. The procedure involves adding standards and samples to wells, applying detection reagents, and reading absorbance to interpolate concentrations from a generated standard curve. Reagents should be refrigerated between uses to preserve activity through the kit's shelf life, and components are intended for research use only. Skeletal muscle investigators also apply the kit to examine fiber-type metabolism, exercise adaptation, and injury responses, since FABP3 expression correlates with oxidative capacity in rat tissue.