Rat Factor V/F5 ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables quantitative detection of Factor V (F5) in rat samples. Factor V is an important coagulation protein with a vital role in the blood clotting process, and the kit is intended for research into coagulation disorders, thrombosis, and related conditions in rat models.

The double-antibody sandwich assay is a colorimetric ELISA with a reported sensitivity of 1.875 ng/mL and a working range of 3.125–200 ng/mL. Validated sample types include plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Kits are stored at 2–8°C for up to 12 months.

Reported linearity for EDTA plasma (n=5) ranges from 82% to 101% across 1:2, 1:4, and 1:8 dilutions, while heparin plasma (n=5) ranges from 82% to 100% across the same dilutions. Average recovery is 98% for both EDTA plasma (range 87–103%) and heparin plasma (range 93–104%). The procedure includes binding steps at 37°C, TMB-based color development, and absorbance reading at 450 nm.